Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning
Joint Authors
Liu, Hao
Sugawara, Michiko
Miyoshi, Hiromi
Miura, Takuya
Tanaka, Hiroto
Tsubota, Ken-ichi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-29
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
To understand the mechanism regulating the spontaneous change in polarity that leads to cell turning, we quantitatively analyzed the dynamics of focal adhesions (FAs) coupling with the self-assembling actin cytoskeletal structure in Swiss 3T3 fibroblasts.
Fluorescent images were acquired from cells expressing GFP-actin and RFP-zyxin by laser confocal microscopy.
On the basis of the maximum area, duration, and relocation distance of FAs extracted from the RFP-zyxin images, the cells could be divided into 3 regions: the front region, intermediate lateral region, and rear region.
In the intermediate lateral region, FAs appeared close to the leading edge and were stabilized gradually as its area increased.
Simultaneously, bundled actin stress fibers (SFs) were observed vertically from the positions of these FAs, and they connected to the other SFs parallel to the leading edge.
Finally, these connecting SFs fused to form a single SF with matured FAs at both ends.
This change in SF organization with cell retraction in the first cycle of migration followed by a newly formed protrusion in the next cycle is assumed to lead to cell turning in migrating Swiss 3T3 fibroblasts.
American Psychological Association (APA)
Sugawara, Michiko& Miyoshi, Hiromi& Miura, Takuya& Tanaka, Hiroto& Tsubota, Ken-ichi& Liu, Hao. 2016. Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning. BioMed Research International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1098205
Modern Language Association (MLA)
Sugawara, Michiko…[et al.]. Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning. BioMed Research International No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1098205
American Medical Association (AMA)
Sugawara, Michiko& Miyoshi, Hiromi& Miura, Takuya& Tanaka, Hiroto& Tsubota, Ken-ichi& Liu, Hao. Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1098205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098205