Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells
Joint Authors
Zhou, Xian
Razmovski-Naumovski, Valentina
Chang, Dennis
Kam, Antony
Low, Mitchell
Chan, Kelvin
Bensoussan, Alan
Li, Chun Guang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Aims.
This study aims to investigate the possible synergistic interactions of the Danshen-Sanqi combination on vascular disease via their anti-inflammatory activities.
Methods.
Nine combination ratios of Danshen-Sanqi extracts were screened in the RAW264.7 cell line and their anti-inflammatory effects were examined in lipopolysaccharide- (LPS-) induced nitric oxide (NO), tumor necrosis factor (TNF), and monocyte chemoattractant protein-1 (MCP-1) generation pathways.
The interaction between Danshen and Sanqi on each target was analysed using combination index (CI) and isobologram models.
Additionally, the anti-inflammatory activities of key bioactive compounds from Danshen and Sanqi were tested using the same models.
The compounds from each herb that exerted the most potent activity were combined to evaluate their possible synergistic/antagonistic interactions.
Results.
Danshen-Sanqi 8 : 2 was found to be the optimal ratio and exerted a synergistic effect in inhibiting NO, TNF, and MCP-1 when the concentrations were higher than 1.24, 1.89, and 2.17 mg/mL, respectively.
Although dihydrotanshinone I (DT) and ginsenoside Rd (Rd) from Danshen and Sanqi, respectively, exhibited the greatest individual bioactivity in the assays, antagonistic effects were observed for the DT-Rd combination 7 : 3.
Conclusion.
This study provided scientific evidence to support the traditional use of the Danshen-Sanqi combination for vascular disease through their synergistic interactions on anti-inflammatory pathways.
American Psychological Association (APA)
Zhou, Xian& Razmovski-Naumovski, Valentina& Chang, Dennis& Li, Chun Guang& Kam, Antony& Low, Mitchell…[et al.]. 2016. Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1098213
Modern Language Association (MLA)
Zhou, Xian…[et al.]. Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1098213
American Medical Association (AMA)
Zhou, Xian& Razmovski-Naumovski, Valentina& Chang, Dennis& Li, Chun Guang& Kam, Antony& Low, Mitchell…[et al.]. Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1098213
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098213