N-Methyl-D-aspartate Receptor Excessive Activation Inhibited Fetal Rat Lung Development In Vivo and In Vitro
Joint Authors
Liao, Zhengchang
Zhou, Xiaocheng
Luo, Ziqiang
Huo, Huiyi
Wang, Mingjie
Yu, Xiaohe
Cao, Chuanding
Ding, Ying
Xiong, Zeng
Yue, Shaojie
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Intrauterine hypoxia is a common cause of fetal growth and lung development restriction.
Although N-methyl-D-aspartate receptors (NMDARs) are distributed in the postnatal lung and play a role in lung injury, little is known about NMDAR’s expression and role in fetal lung development.
Methods.
Real-time PCR and western blotting analysis were performed to detect NMDARs between embryonic days (E) 15.5 and E21.5 in fetal rat lungs.
NMDAR antagonist MK-801’s influence on intrauterine hypoxia-induced retardation of fetal lung development was tested in vivo, and NMDA’s direct effect on fetal lung development was observed using fetal lung organ culture in vitro.
Results.
All seven NMDARs are expressed in fetal rat lungs.
Intrauterine hypoxia upregulated NMDARs expression in fetal lungs and decreased fetal body weight, lung weight, lung-weight-to-body-weight ratio, and radial alveolar count, whereas MK-801 alleviated this damage in vivo.
In vitro experiments showed that NMDA decreased saccular circumference and area per unit and downregulated thyroid transcription factor-1 and surfactant protein-C mRNA expression.
Conclusions.
The excessive activation of NMDARs contributed to hypoxia-induced fetal lung development retardation and appropriate blockade of NMDAR might be a novel therapeutic strategy for minimizing the negative outcomes of prenatal hypoxia on lung development.
American Psychological Association (APA)
Liao, Zhengchang& Zhou, Xiaocheng& Luo, Ziqiang& Huo, Huiyi& Wang, Mingjie& Yu, Xiaohe…[et al.]. 2016. N-Methyl-D-aspartate Receptor Excessive Activation Inhibited Fetal Rat Lung Development In Vivo and In Vitro. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098231
Modern Language Association (MLA)
Liao, Zhengchang…[et al.]. N-Methyl-D-aspartate Receptor Excessive Activation Inhibited Fetal Rat Lung Development In Vivo and In Vitro. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1098231
American Medical Association (AMA)
Liao, Zhengchang& Zhou, Xiaocheng& Luo, Ziqiang& Huo, Huiyi& Wang, Mingjie& Yu, Xiaohe…[et al.]. N-Methyl-D-aspartate Receptor Excessive Activation Inhibited Fetal Rat Lung Development In Vivo and In Vitro. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098231
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098231