Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method
Joint Authors
Cheng, Tao
Yang, Jun
Zhang, Tong
Yang, Yi-Shun
Ding, Yue
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A crude β-glucosidase has been produced from Trichoderma viride and used to explore a simple method to prepare icariside II from icariin.
The crude enzyme has been studied by zymography method and used for hydrolysis of ICA.
To achieve a high conversion rate of ICA, various factors have been studied including pH, reaction time, temperature, initial concentration of enzyme, and initial concentration of ICA through central composite design experiments.
In the condition of the optimum hydrolysis parameters with pH 4.0, 41°C, 1.0 mg/mL ICA, and 9.8 U/mL crude β-glucosidase, the conversion rate of ICA reached 95.03% at 1 h.
Moreover, the cytotoxicity test showed that ICA II performed inhibition effects on proliferation of A549 cell, while ICA has no cytotoxicity.
It indicated that the hydrolysis transformation study of ICA is valuable for exploration of active new drugs.
American Psychological Association (APA)
Cheng, Tao& Yang, Jun& Zhang, Tong& Yang, Yi-Shun& Ding, Yue. 2016. Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098273
Modern Language Association (MLA)
Cheng, Tao…[et al.]. Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1098273
American Medical Association (AMA)
Cheng, Tao& Yang, Jun& Zhang, Tong& Yang, Yi-Shun& Ding, Yue. Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098273