Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris
Joint Authors
Karim, Kazi Muhammad Rezaul
Husaini, Ahmad
Sing, Ngieng Ngui
Mohd Sinang, Fazia
Hussain, Mohd Hasnain Md.
Hossain, Md. Anowar
Roslan, Hairul Azman
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A novel thermostable glucoamylase cDNA without starch binding domain (SBD) of Aspergillus flavus NSH9 was successfully identified, isolated, and overexpressed in Pichia pastoris GS115.
The complete open reading frame of glucoamylase from Aspergillus flavus NSH9 was identified by employing PCR that encodes 493 amino acids lacking in the SBD.
The first 17 amino acids were presumed to be a signal peptide.
The cDNA was cloned into Pichia pastoris and the highest expression of recombinant glucoamylase (rGA) was observed after 8 days of incubation period with 1% methanol.
The molecular weight of the purified rGA was about 78 kDa and exhibited optimum catalytic activity at pH 5.0 and temperature of 70°C.
The enzyme was stable at higher temperature with 50% of residual activity observed after 20 min at 90°C and 100°C.
Low concentration of metal (Mg++, Fe++, Zn++, Cu++, and Pb++) had positive effect on rGA activity.
This rGA has the potential for use and application in the saccharification steps, due to its thermostability, in the starch processing industries.
American Psychological Association (APA)
Karim, Kazi Muhammad Rezaul& Husaini, Ahmad& Hossain, Md. Anowar& Sing, Ngieng Ngui& Mohd Sinang, Fazia& Hussain, Mohd Hasnain Md.…[et al.]. 2016. Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098290
Modern Language Association (MLA)
Karim, Kazi Muhammad Rezaul…[et al.]. Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1098290
American Medical Association (AMA)
Karim, Kazi Muhammad Rezaul& Husaini, Ahmad& Hossain, Md. Anowar& Sing, Ngieng Ngui& Mohd Sinang, Fazia& Hussain, Mohd Hasnain Md.…[et al.]. Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098290