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Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific Oyster Crassostrea gigas
Joint Authors
Li, Xing-Xia
Yu, Wen-Chao
Cai, Zhong-Qiang
He, Cheng
Wei, Na
Wang, Xiao-Tong
Yue, Xi-Qing
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The shell of the pearl oyster (Pinctada fucata) mainly comprises aragonite whereas that of the Pacific oyster (Crassostrea gigas) is mainly calcite, thereby suggesting the different mechanisms of shell formation between above two mollusks.
Calmodulin (CaM) is an important gene for regulating the uptake, transport, and secretion of calcium during the process of shell formation in pearl oyster.
It is interesting to characterize the CaM in oysters, which could facilitate the understanding of the different shell formation mechanisms among mollusks.
We cloned the full-length cDNA of Pacific oyster CaM (cgCaM) and found that the cgCaM ORF encoded a peptide of 113 amino acids containing three EF-hand calcium-binding domains, its expression level was highest in the mantle, hinting that the cgCaM gene is probably involved in shell formation of Pacific oyster, and the common ancestor of Gastropoda and Bivalvia may possess at least three CaM genes.
We also found that the numbers of some EF hand family members in highly calcified species were higher than those in lowly calcified species and the numbers of these motifs in oyster genome were the highest among the mollusk species with whole genome sequence, further hinting the correlation between CaM and biomineralization.
American Psychological Association (APA)
Li, Xing-Xia& Yu, Wen-Chao& Cai, Zhong-Qiang& He, Cheng& Wei, Na& Wang, Xiao-Tong…[et al.]. 2016. Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific Oyster Crassostrea gigas. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098327
Modern Language Association (MLA)
Li, Xing-Xia…[et al.]. Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific Oyster Crassostrea gigas. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098327
American Medical Association (AMA)
Li, Xing-Xia& Yu, Wen-Chao& Cai, Zhong-Qiang& He, Cheng& Wei, Na& Wang, Xiao-Tong…[et al.]. Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific Oyster Crassostrea gigas. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098327
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098327