Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells
Joint Authors
Finoli, Anthony
Schmelzer, Eva
Over, Patrick
Nettleship, Ian
Gerlach, Joerg C.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Liver cell culture within three-dimensional structures provides an improved culture system for various applications in basic research, pharmacological screening, and implantable or extracorporeal liver support.
Biodegradable calcium-based scaffolds in such systems could enhance liver cell functionality by providing endothelial and hepatic cell support through locally elevated calcium levels, increased surface area for cell attachment, and allowing three-dimensional tissue restructuring.
Open-porous hydroxyapatite scaffolds were fabricated and seeded with primary adult human liver cells, which were embedded within or without gels of extracellular matrix protein collagen-1 or hyaluronan.
Metabolic functions were assessed after 5, 15, and 28 days.
Longer-term cultures exhibited highest cell numbers and liver specific gene expression when cultured on hydroxyapatite scaffolds in collagen-1.
Endothelial gene expression was induced in cells cultured on scaffolds without extracellular matrix proteins.
Hydroxyapatite induced gene expression for cytokeratin-19 when cells were cultured in collagen-1 gel while culture in hyaluronan increased cytokeratin-19 gene expression independent of the use of scaffold in long-term culture.
The implementation of hydroxyapatite composites with extracellular matrices affected liver cell cultures and cell differentiation depending on the type of matrix protein and the presence of a scaffold.
The hydroxyapatite scaffolds enable scale-up of hepatic three-dimensional culture models for regenerative medicine applications.
American Psychological Association (APA)
Finoli, Anthony& Schmelzer, Eva& Over, Patrick& Nettleship, Ian& Gerlach, Joerg C.. 2016. Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098351
Modern Language Association (MLA)
Finoli, Anthony…[et al.]. Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098351
American Medical Association (AMA)
Finoli, Anthony& Schmelzer, Eva& Over, Patrick& Nettleship, Ian& Gerlach, Joerg C.. Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098351
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098351