The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models
Joint Authors
Pomothy, Judit
Szombath, Gergely
Rokonál, Patrik
Mátis, Gábor
Neogrády, Zsuzsanna
Steinmetzer, Torsten
Pászti-Gere, Erzsébet
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
Dysfunction of matriptase-2 can be involved in iron regulatory disorder via downregulation of hepcidin expression.
In the present study, we investigated the effects of 3-amidinophenylalanine-derived matriptase inhibitors on porcine hepatic inflammatory cell models.
Methods.
Hepatocyte-Kupffer cell cocultures (ratio of 2 : 1 and 6 : 1) were treated with four structurally related matriptase inhibitors at 50 μM.
Cell cytotoxicity and relative expressions of IL-6 and IL-8 and the levels of hepcidin were determined by MTS and porcine-specific ELISA.
The extracellular H2O2 contents were analyzed by Amplex Red method.
Results.
Matriptase inhibitors at 50 µM for 24 h did not increase cell death rate.
The elevated ROS production observed after short-term application of inhibitor MI-441 could be correlated with lowered hepcidin expression.
MI-460 could significantly enhance hepcidin levels in the supernatants of cocultures (by 62.21 ± 26.8 % in hepatocyte-Kupffer cell, 2 : 1, and by 42.6 ± 14.3 % in hepatocyte-Kupffer cell, 6 : 1, cocultures, resp.).
No significant changes were found in IL-6 and IL-8 levels in cocultures exposed to matriptase inhibitors.
Conclusions.
Based on in vitro findings, administration of MI-460 via modulation of hepcidin expression without cytotoxic and oxidative stress inducing properties might be a reliable alternative to treat iron overload in human and veterinary clinical practice.
American Psychological Association (APA)
Pomothy, Judit& Szombath, Gergely& Rokonál, Patrik& Mátis, Gábor& Neogrády, Zsuzsanna& Steinmetzer, Torsten…[et al.]. 2016. The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098431
Modern Language Association (MLA)
Pomothy, Judit…[et al.]. The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098431
American Medical Association (AMA)
Pomothy, Judit& Szombath, Gergely& Rokonál, Patrik& Mátis, Gábor& Neogrády, Zsuzsanna& Steinmetzer, Torsten…[et al.]. The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098431
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098431