Different Levels of DNA Methylation Detected in Human Sperms after Morphological Selection Using High Magnification Microscopy
Joint Authors
Copin, Henri
Montjean, Debbie
Siffroi, Jean-Pierre
Bouret, Dominique
Marzouk, Flora
Benkhalifa, Moncef
Cassuto, Nino Guy
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To analyze DNA methylation levels between two groups of spermatozoa taken from the same sample, following morphological selection by high magnification (HM) at 6100x microscopy.
A prospective study was conducted and studied 876 spermatozoa from 10 randomly selected men.
Sperm morphology was characterized at HM according to criteria previously established.
High-scoring Score 6 and low-scoring Score 0 sperm were selected.
Sperm DNA methylation level was assessed using an immunoassay method targeting 5-methylcytosine residues by fluorescence microscopy with imaging analysis system to detect DNA methylation in single spermatozoon.
Results.
In total, 448 S6 spermatozoa and 428 S0 spermatozoa were analyzed.
A strong relationship was found between sperm DNA methylation levels and sperm morphology observed at HM.
Sperm DNA methylation level in the S6 group was significantly lower compared with that in the S0 group ( p < 10 - 6 ), OR = 2.4; and p < 0.001 , as determined using the Wilcoxon test.
Conclusion.
Differences in DNA methylation levels are associated with sperm morphology variations as observed at HM, which allows spermatozoa with abnormal levels to be discarded and ultimately decrease birth defects, malformations, and epigenetic diseases that may be transmitted from sperm to offspring in ICSI.
American Psychological Association (APA)
Cassuto, Nino Guy& Montjean, Debbie& Siffroi, Jean-Pierre& Bouret, Dominique& Marzouk, Flora& Copin, Henri…[et al.]. 2016. Different Levels of DNA Methylation Detected in Human Sperms after Morphological Selection Using High Magnification Microscopy. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098442
Modern Language Association (MLA)
Cassuto, Nino Guy…[et al.]. Different Levels of DNA Methylation Detected in Human Sperms after Morphological Selection Using High Magnification Microscopy. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098442
American Medical Association (AMA)
Cassuto, Nino Guy& Montjean, Debbie& Siffroi, Jean-Pierre& Bouret, Dominique& Marzouk, Flora& Copin, Henri…[et al.]. Different Levels of DNA Methylation Detected in Human Sperms after Morphological Selection Using High Magnification Microscopy. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098442
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098442