Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases
Joint Authors
Cheng, Lifeng
Duan, Shengwen
Feng, Xiangyuan
Zheng, Ke
Yang, Qi
Liu, Zhengchu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
β -mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation.
This study separated and purified the β -mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a “two-step” method comprising ultrafiltration and gel chromatography.
The purified β -mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg).
The optimum temperature and pH were 65°C and 6.0, respectively.
Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4.5–7.0.
The β -mannanase activity was significantly enhanced in the presence of Mn2+, Cu2+, Zn2+, Ca2+, Mg2+, and Al3+ and strongly inhibited by Ba2+ and Pb2+.
K m and V m a x values for locust bean gum were 7.14 mg/mL and 107.5 μmol/min/mL versus 1.749 mg/mL and 33.45 µmol/min/mL for Konjac glucomannan, respectively.
Therefore, β -mannanase purified by this work shows stability at high temperatures and in weakly acidic or neutral environments.
Based on such data, the β -mannanase will have potential applications as a dietary supplement in treatment of inflammatory processes.
American Psychological Association (APA)
Cheng, Lifeng& Duan, Shengwen& Feng, Xiangyuan& Zheng, Ke& Yang, Qi& Liu, Zhengchu. 2016. Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098445
Modern Language Association (MLA)
Cheng, Lifeng…[et al.]. Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098445
American Medical Association (AMA)
Cheng, Lifeng& Duan, Shengwen& Feng, Xiangyuan& Zheng, Ke& Yang, Qi& Liu, Zhengchu. Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098445
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098445