Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products
Joint Authors
Umezawa, Akihiro
Nasu, Michiyo
Takayama, Shinichiro
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The cell-based therapy for cartilage or bone requires a large number of cells; serial passages of chondrocytes are, therefore, needed.
However, fates of expanded chondrocytes from extra fingers remain unclarified.
The chondrocytes from human epiphyses morphologically changed from small polygonal cells to bipolar elongated spindle cells and to large polygonal cells with degeneration at early passages.
Gene of type II collagen was expressed in the cells only at a primary culture (Passage 0) and Passage 1 (P1) cells.
The nodules by implantation of P0 to P8 cells were composed of cartilage and perichondrium.
The cartilage consisted of chondrocytes with round nuclei and type II collagen-positive matrix, and the perichondrium consisted of spindle cells with type I collage-positive matrix.
The cartilage and perichondrium developed to bone with marrow cavity through enchondral ossification.
Chondrogenesis and osteogenesis by epiphyseal chondrocytes depended on replication number in culture.
It is noteworthy to take population doubling level in correlation with pharmaceutical efficacy into consideration when we use chondrocytes for cell-based therapies.
American Psychological Association (APA)
Nasu, Michiyo& Takayama, Shinichiro& Umezawa, Akihiro. 2016. Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products. BioMed Research International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1098480
Modern Language Association (MLA)
Nasu, Michiyo…[et al.]. Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products. BioMed Research International No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1098480
American Medical Association (AMA)
Nasu, Michiyo& Takayama, Shinichiro& Umezawa, Akihiro. Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1098480
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098480