Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules
Joint Authors
Cavallo, Carola
Mariani, Erminia
Merli, Giulia
Filardo, Giuseppe
Marcacci, Maurilio
Roffi, Alice
Pratelli, Loredana
Kon, Elizaveta
Grigolo, Brunella
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs).
Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing.
Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2 + thrombin, and 10% of collagen type I.
Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α.
PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed.
Collagen type I produced an overall lower GF release.
Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF- β 1 that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours.
CaCl2 induced a progressive release of GFs from 15 minutes and increasing up to 24 hours.
The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.
American Psychological Association (APA)
Cavallo, Carola& Roffi, Alice& Grigolo, Brunella& Mariani, Erminia& Pratelli, Loredana& Merli, Giulia…[et al.]. 2016. Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098518
Modern Language Association (MLA)
Cavallo, Carola…[et al.]. Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098518
American Medical Association (AMA)
Cavallo, Carola& Roffi, Alice& Grigolo, Brunella& Mariani, Erminia& Pratelli, Loredana& Merli, Giulia…[et al.]. Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098518