Molecular Typing Characteristic and Drug Susceptibility Analysis of Mycobacterium tuberculosis Isolates from Zigong, China
Joint Authors
Wan, Kanglin
Jiang, Yi
Zhao, Xiuqin
Dong, Haiyan
Liu, Haican
Deng, Jian-Ping
Xiao, Ti-Quan
Zhang, Zheng-Dong
Liu, Zhi-Guang
Li, Qun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
China is one of the 22 countries with high TB burden worldwide, and Sichuan contained the second-largest number of TB cases among all of the Chinese provinces.
But the characteristics of Mycobacterium tuberculosis circulated in Zigong, Sichuan, were still unknown.
To investigate the character and drug resistance profile, 265 clinical isolates were cultured from tuberculosis patient’s sputum samples in the year of 2010, of which the genetic profile was determined by using Spoligotyping and MIRU-VNTR typing methods, and the drug sensibility testing to the four first-line and four second-line antituberculosis (anti-TB) drugs was performed by using proportion method on Lowenstein-Jensen (L-J) media.
The major Spoligotype was Beijing family (143/265, 53.96%), followed by T (80/265, 30.19%) and H (9/265, 3.40%) genotypes; the total Hunter-Gaston discrimination index (HGDI) of the 24 loci MIRU-VNTR was 0.9995.
About 27.17% (72/265) of the isolates were resistant to at least one of the eight tested anti-TB drugs, and for Beijing and non-Beijing family isolates the proportion of drug resistance was 28.47% (41/144) and 25.62% (31/121), respectively.
That is, the most prevalent genotype here was Beijing family, and the 24 loci VNTR analysis could supply a high resolution for genotyping, and Beijing and non-Beijing isolates had no difference (p>0.05) for drug resistance.
American Psychological Association (APA)
Liu, Haican& Deng, Jian-Ping& Dong, Haiyan& Xiao, Ti-Quan& Zhao, Xiuqin& Zhang, Zheng-Dong…[et al.]. 2016. Molecular Typing Characteristic and Drug Susceptibility Analysis of Mycobacterium tuberculosis Isolates from Zigong, China. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098552
Modern Language Association (MLA)
Liu, Haican…[et al.]. Molecular Typing Characteristic and Drug Susceptibility Analysis of Mycobacterium tuberculosis Isolates from Zigong, China. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098552
American Medical Association (AMA)
Liu, Haican& Deng, Jian-Ping& Dong, Haiyan& Xiao, Ti-Quan& Zhao, Xiuqin& Zhang, Zheng-Dong…[et al.]. Molecular Typing Characteristic and Drug Susceptibility Analysis of Mycobacterium tuberculosis Isolates from Zigong, China. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098552
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098552