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Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts
Joint Authors
Li, Jianbo
Bao, Baoliang
Liu, Lei
Wang, Xuemei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Near-infrared fluorescence (NIRF) imaging technology is a highly sensitive imaging modality and has been widely used in noninvasively studying the status of receptor expression in small animal models, with an appropriate NIRF probe targeting a specific receptor.
In this report, Cy5.5-conjugated anti-CAIX monoclonal antibody (Mab-Cy5.5) was evaluated in athymic mice bearing HT-29 tumor xenografts in order to investigate the effect of conjugate on tumor targeting efficacy.
In vitro binding studies showed that Mab-Cy5.5 could specifically bind to the cells which expressed CAIX.
Results from in vivo imaging showed that HT-29 tumor xenografts can be clearly visualized at 48 h after injection of Mab-Cy5.5, and in the blocking experiment, free anti-CAIX antibody effectively blocked the concentration of Mab-Cy5.5 in the tumors.
Western blotting and immunohistochemistry analysis of HT-29 tumor xenografts verified the expression of CAIX in HT-29 tumors.
Mab-Cy5.5 could specifically bind to the tumors which expressed CAIX.
These results suggested that Mab-Cy5.5 was suitable for CAIX expression imaging in the preclinical research.
American Psychological Association (APA)
Li, Jianbo& Bao, Baoliang& Liu, Lei& Wang, Xuemei. 2016. Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098604
Modern Language Association (MLA)
Li, Jianbo…[et al.]. Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098604
American Medical Association (AMA)
Li, Jianbo& Bao, Baoliang& Liu, Lei& Wang, Xuemei. Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098604
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098604