Histone Lysine Methylation in TGF-β1 Mediated p21 Gene Expression in Rat Mesangial Cells
Joint Authors
Sun, Guang-dong
Guo, Qiao-yan
Li, Xiaoxia
Han, Hongbo
Li, Chaoyuan
Liu, Shujun
Gao, Wenhui
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Transforming growth factor beta1- (TGF-β1-) induced p21-dependent mesangial cell (MC) hypertrophy plays a key role in the pathogenesis of chronic renal diseases including diabetic nephropathy (DN).
Increasing evidence demonstrated the role of posttranscriptional modifications (PTMs) in the event; however, the precise regulatory mechanism of histone lysine methylation remains largely unknown.
Here, we examined the roles of both histone H3 lysine 4 and lysine 9 methylations (H3K4me/H3K9me) in TGF-β1 induced p21 gene expression in rat mesangial cells (RMCs).
Our results indicated that TGF-β1 upregulated the expression of p21 gene in RMCs, which was positively correlated with the increased chromatin marks associated with active genes (H3K4me1/H3K4me2/H3K4me3) and negatively correlated with the decreased levels of repressive marks (H3K9me2/H3K9me3) at p21 gene promoter.
TGF-β1 also elevated the recruitment of the H3K4 methyltransferase (HMT) SET7/9 to the p21 gene promoter.
SET7/9 gene silencing with small interfering RNAs (siRNAs) significantly abolished the TGF-β1 induced p21 gene expression.
Taken together, these results reveal the key role of histone H3Kme in TGF-β1 mediated p21 gene expression in RMC, partly through HMT SET7/9 occupancy, suggesting H3Kme and SET7/9 may be potential renoprotective agents in managing chronic renal diseases.
American Psychological Association (APA)
Guo, Qiao-yan& Li, Xiaoxia& Han, Hongbo& Li, Chaoyuan& Liu, Shujun& Gao, Wenhui…[et al.]. 2016. Histone Lysine Methylation in TGF-β1 Mediated p21 Gene Expression in Rat Mesangial Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098629
Modern Language Association (MLA)
Guo, Qiao-yan…[et al.]. Histone Lysine Methylation in TGF-β1 Mediated p21 Gene Expression in Rat Mesangial Cells. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098629
American Medical Association (AMA)
Guo, Qiao-yan& Li, Xiaoxia& Han, Hongbo& Li, Chaoyuan& Liu, Shujun& Gao, Wenhui…[et al.]. Histone Lysine Methylation in TGF-β1 Mediated p21 Gene Expression in Rat Mesangial Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098629