Influence of Botulinumtoxin A on the Expression of Adult MyHC Isoforms in the Masticatory Muscles in Dystrophin-Deficient Mice (Mdx-Mice)
Joint Authors
Kunert-Keil, Christiane
Botzenhart, Ute Ulrike
Wegenstein, Constantin
Todorov, Teodor
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The most widespread animal model to investigate Duchenne muscular dystrophy is the mdx-mouse.
In contrast to humans, phases of muscle degeneration are replaced by regeneration processes; hence there is only a restricted time slot for research.
The aim of the study was to investigate if an intramuscular injection of BTX-A is able to break down muscle regeneration and has direct implications on the gene expression of myosin heavy chains in the corresponding treated and untreated muscles.
Therefore, paralysis of the right masseter muscle was induced in adult healthy and dystrophic mice by a specific intramuscular injection of BTX-A.
After 21 days the mRNA expression and protein content of MyHC isoforms of the right and left masseter, temporal, and the tongue muscle were determined using quantitative RT-PCR and Western blot technique.
MyHC-IIa and MyHC-I-mRNA expression significantly increased in the paralyzed masseter muscle of control-mice, whereas MyHC-IIb and MyHC-IIx/d-mRNA were decreased.
In dystrophic muscles no effect of BTX-A could be detected at the level of MyHC.
This study suggests that BTX-A injection is a suitable method to simulate DMD-pathogenesis in healthy mice but further investigations are necessary to fully analyse the BTX-A effect and to generate sustained muscular atrophy in mdx-mice.
American Psychological Association (APA)
Botzenhart, Ute Ulrike& Wegenstein, Constantin& Todorov, Teodor& Kunert-Keil, Christiane. 2016. Influence of Botulinumtoxin A on the Expression of Adult MyHC Isoforms in the Masticatory Muscles in Dystrophin-Deficient Mice (Mdx-Mice). BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098662
Modern Language Association (MLA)
Botzenhart, Ute Ulrike…[et al.]. Influence of Botulinumtoxin A on the Expression of Adult MyHC Isoforms in the Masticatory Muscles in Dystrophin-Deficient Mice (Mdx-Mice). BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1098662
American Medical Association (AMA)
Botzenhart, Ute Ulrike& Wegenstein, Constantin& Todorov, Teodor& Kunert-Keil, Christiane. Influence of Botulinumtoxin A on the Expression of Adult MyHC Isoforms in the Masticatory Muscles in Dystrophin-Deficient Mice (Mdx-Mice). BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098662