Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform
Joint Authors
Umezawa, Akihiro
Tone, Hiroshi
Yoshioka, Saeko
Akiyama, Hirokazu
Nishimura, Akira
Ichimura, Masaki
Nakatani, Masaru
Kiyono, Tohru
Watanabe, Masatoshi
Toyoda, Masashi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Automation of cell culture would facilitate stable cell expansion with consistent quality.
In the present study, feasibility of an automated closed-cell culture system “P 4C S” for an embryoid body- (EB-) explant outgrowth culture was investigated as a model case for explant culture.
After placing the induced pluripotent stem cell- (iPSC-) derived EBs into the system, the EBs successfully adhered to the culture surface and the cell outgrowth was clearly observed surrounding the adherent EBs.
After confirming the outgrowth, we carried out subculture manipulation, in which the detached cells were simply dispersed by shaking the culture flask, leading to uniform cell distribution.
This enabled continuous stable cell expansion, resulting in a cell yield of 3.1 × 107.
There was no evidence of bacterial contamination throughout the cell culture experiments.
We herewith developed the automated cultivation platform for EB-explant outgrowth cells.
American Psychological Association (APA)
Tone, Hiroshi& Yoshioka, Saeko& Akiyama, Hirokazu& Nishimura, Akira& Ichimura, Masaki& Nakatani, Masaru…[et al.]. 2016. Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098715
Modern Language Association (MLA)
Tone, Hiroshi…[et al.]. Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098715
American Medical Association (AMA)
Tone, Hiroshi& Yoshioka, Saeko& Akiyama, Hirokazu& Nishimura, Akira& Ichimura, Masaki& Nakatani, Masaru…[et al.]. Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098715