A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs
Joint Authors
Mai, Guoqin
Ge, Ruiquan
Sun, Guoquan
Meng, Qinghan
Zhou, Fengfeng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Motivation.
Clustered regularly interspaced short palindromic repeat (CRISPR) is a genetic element with active regulation roles for foreign invasive genes in the prokaryotic genomes and has been engineered to work with the CRISPR-associated sequence (Cas) gene Cas9 as one of the modern genome editing technologies.
Due to inconsistent definitions, the existing CRISPR detection programs seem to have missed some weak CRISPR signals.
Results.
This study manually curates all the currently annotated CRISPR elements in the prokaryotic genomes and proposes 95 updates to the annotations.
A new definition is proposed to cover all the CRISPRs.
The comprehensive comparison of CRISPR numbers on the taxonomic levels of both domains and genus shows high variations for closely related species even in the same genus.
The detailed investigation of how CRISPRs are evolutionarily manipulated in the 8 completely sequenced species in the genus Thermoanaerobacter demonstrates that transposons act as a frequent tool for splitting long CRISPRs into shorter ones along a long evolutionary history.
American Psychological Association (APA)
Mai, Guoqin& Ge, Ruiquan& Sun, Guoquan& Meng, Qinghan& Zhou, Fengfeng. 2016. A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098747
Modern Language Association (MLA)
Mai, Guoqin…[et al.]. A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098747
American Medical Association (AMA)
Mai, Guoqin& Ge, Ruiquan& Sun, Guoquan& Meng, Qinghan& Zhou, Fengfeng. A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098747
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098747