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Overexpression of AaWRKY1 Leads to an Enhanced Content of Artemisinin in Artemisia annua
Joint Authors
Wang, Guo-Feng
Fu, Xueqing
Lv, Zongyou
Shen, Qian
Yan, Tingxian
Jiang, Weiming
Sun, Xiaofen
Zhang, Lida
Pan, Qifang
Tang, Yueli
Shi, Pu
Li, Ling
Tang, Ke-Xuan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Artemisinin is an effective component of drugs against malaria.
The regulation of artemisinin biosynthesis is at the forefront of artemisinin research.
Previous studies showed that AaWRKY1 can regulate the expression of ADS, which is the first key enzyme in artemisinin biosynthetic pathway.
In this study, AaWRKY1 was cloned, and it activated ADSpro and CYPpro in tobacco using dual-LUC assay.
To further study the function of AaWRKY1, pCAMBIA2300-AaWRKY1 construct under 35S promoter was generated.
Transgenic plants containing AaWRKY1 were obtained, and four independent lines with high expression of AaWRKY1 were analyzed.
The expression of ADS and CYP, the key enzymes in artemisinin biosynthetic pathway, was dramatically increased in AaWRKY1-overexpressing A.
annua plants.
Furthermore, the artemisinin yield increased significantly in AaWRKY1-overexpressing A.
annua plants.
These results showed that AaWRKY1 increased the content of artemisinin by regulating the expression of both ADS and CYP.
It provides a new insight into the mechanism of regulation on artemisinin biosynthesis via transcription factors in the future.
American Psychological Association (APA)
Jiang, Weiming& Fu, Xueqing& Pan, Qifang& Tang, Yueli& Shen, Qian& Lv, Zongyou…[et al.]. 2016. Overexpression of AaWRKY1 Leads to an Enhanced Content of Artemisinin in Artemisia annua. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098768
Modern Language Association (MLA)
Jiang, Weiming…[et al.]. Overexpression of AaWRKY1 Leads to an Enhanced Content of Artemisinin in Artemisia annua. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098768
American Medical Association (AMA)
Jiang, Weiming& Fu, Xueqing& Pan, Qifang& Tang, Yueli& Shen, Qian& Lv, Zongyou…[et al.]. Overexpression of AaWRKY1 Leads to an Enhanced Content of Artemisinin in Artemisia annua. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098768