Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles
Joint Authors
Wang, Guannan
Liu, Yaxu
Zhang, Xuanjun
E, Yifeng
Fang, Fang
Kuang, Guangkai
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A novel magnetic fluorescent encoded nanoimmunoassay system for multicomponent detection and separation of the subtrace pathogenic DNA (hepatitis B virus surface gene, HBV; hepatitis A virus poly the protein gene, HAV) was established based on new type of magnetic fluorescent encoded nanoparticles and sandwich immunoassay principle.
This method combines multifunctional nanoparticles, immunoassay technique, fluorescence labeling, and magnetic separation of multicomponent technology.
It has many advantages such as high sensitivity, low detection limit, easy operation, and great potential for development.
The results of this work show that, based on nanoimmunoassay system, it could quantitatively detect the multicomponent trace pathogenic HAV and HBV DNA, as well as detection limit up to 0.1 pM and 0.12 pM.
Furthermore, with the improvement of the performances of magnetic fluorescent encoded nanoparticles, the sensitivity will be further improved.
In this experiment, a new nanoimmunoassay system based on magnetic fluorescent encoded nanoparticles was established, which will provide a new way for the immunoassay and separation of multicomponent biomolecules.
American Psychological Association (APA)
Liu, Yaxu& Zhang, Xuanjun& E, Yifeng& Fang, Fang& Kuang, Guangkai& Wang, Guannan. 2016. Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098770
Modern Language Association (MLA)
Liu, Yaxu…[et al.]. Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098770
American Medical Association (AMA)
Liu, Yaxu& Zhang, Xuanjun& E, Yifeng& Fang, Fang& Kuang, Guangkai& Wang, Guannan. Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098770