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In Vitro Protective Effects of Lycium barbarum Berries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells
Joint Authors
Villarini, Milena
Moretti, Massimo
Albi, Elisabetta
Cataldi, Samuela
Ceccarini, M. R.
Vannini, S.
Beccari, T.
Codini, M.
Fioretti, Bernard
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Lycium barbarum is a famous plant in the traditional Chinese medicine.
The plant is known to have health-promoting bioactive components.
The properties of Lycium barbarum berries cultivated in Umbria (Italy) and their effect on human hepatocellular carcinoma cells (HepG2) have been investigated in this work.
The obtained results demonstrated that the Lycium barbarum berries from Umbria region display high antioxidant properties evaluated by total phenolic content and ORAC method, on hydrophilic and lipophilic fractions.
Moreover, on HepG2 cell line Lycium barbarum berries extract did not change cell viability analyzed by MTT and Trypan blue exclusion assay and did not induce genotoxic effect analyzed by comet assay.
Furthermore, it was demonstrated, for the first time, that the berries extract showed a protective effect on DNA damage, expressed as antigenotoxic activity in vitro.
Finally, Lycium barbarum berries extract was able to modulate the expression of genes involved in oxidative stress, proliferation, apoptosis, and cancer.
In particular, downexpression of genes involved in tumor migration and invasion (CCL5), in increased risk of metastasis and antiapoptotic signal (DUSP1), and in carcinogenesis (GPx-3 and PTGS1), together with overexpression of tumor suppressor gene (MT3), suggested that Umbrian Lycium barbarum berries could play a protective role against hepatocellular carcinoma.
American Psychological Association (APA)
Ceccarini, M. R.& Vannini, S.& Cataldi, Samuela& Moretti, Massimo& Villarini, Milena& Fioretti, Bernard…[et al.]. 2016. In Vitro Protective Effects of Lycium barbarum Berries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098809
Modern Language Association (MLA)
Ceccarini, M. R.…[et al.]. In Vitro Protective Effects of Lycium barbarum Berries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098809
American Medical Association (AMA)
Ceccarini, M. R.& Vannini, S.& Cataldi, Samuela& Moretti, Massimo& Villarini, Milena& Fioretti, Bernard…[et al.]. In Vitro Protective Effects of Lycium barbarum Berries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098809
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098809