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Identification of Deleterious Mutations in Myostatin Gene of Rohu Carp (Labeo rohita) Using Modeling and Molecular Dynamic Simulation Approaches
Joint Authors
Barman, Hirak Kumar
Rasal, Kiran Dashrath
Chakrapani, Vemulawada
Nayak, Swapnarani
Jena, Sasmita
Sundaray, Jitendra Kumar
Jayasankar, Pallipuram
Mohapatra, Shibani Dutta
Patra, Swagat Kumar
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The myostatin (MSTN) is a known negative growth regulator of skeletal muscle.
The mutated myostatin showed a double-muscular phenotype having a positive significance for the farmed animals.
Consequently, adequate information is not available in the teleosts, including farmed rohu carp, Labeo rohita.
In the absence of experimental evidence, computational algorithms were utilized in predicting the impact of point mutation of rohu myostatin, especially its structural and functional relationships.
The four mutations were generated at different positions (p.D76A, p.Q204P, p.C312Y, and p.D313A) of MSTN protein of rohu.
The impacts of each mutant were analyzed using SIFT, I-Mutant 2.0, PANTHER, and PROVEAN, wherein two substitutions (p.D76A and p.Q204P) were predicted as deleterious.
The comparative structural analysis of each mutant protein with the native was explored using 3D modeling as well as molecular-dynamic simulation techniques.
The simulation showed altered dynamic behaviors concerning RMSD and RMSF, for either p.D76A or p.Q204P substitution, when compared with the native counterpart.
Interestingly, incorporated two mutations imposed a significant negative impact on protein structure and stability.
The present study provided the first-hand information in identifying possible amino acids, where mutations could be incorporated into MSTN gene of rohu carp including other carps for undertaking further in vivo studies.
American Psychological Association (APA)
Rasal, Kiran Dashrath& Chakrapani, Vemulawada& Patra, Swagat Kumar& Mohapatra, Shibani Dutta& Nayak, Swapnarani& Jena, Sasmita…[et al.]. 2016. Identification of Deleterious Mutations in Myostatin Gene of Rohu Carp (Labeo rohita) Using Modeling and Molecular Dynamic Simulation Approaches. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098818
Modern Language Association (MLA)
Rasal, Kiran Dashrath…[et al.]. Identification of Deleterious Mutations in Myostatin Gene of Rohu Carp (Labeo rohita) Using Modeling and Molecular Dynamic Simulation Approaches. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1098818
American Medical Association (AMA)
Rasal, Kiran Dashrath& Chakrapani, Vemulawada& Patra, Swagat Kumar& Mohapatra, Shibani Dutta& Nayak, Swapnarani& Jena, Sasmita…[et al.]. Identification of Deleterious Mutations in Myostatin Gene of Rohu Carp (Labeo rohita) Using Modeling and Molecular Dynamic Simulation Approaches. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098818
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098818