Detection of Natural Toxoplasma gondii Infection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction
Joint Authors
Ngotho, J. M.
Maina, Naomi Wangari
Mose, John Mokua
Karanja, Simon Muturi
Kamau, David Muchina
Njuguna, Adele Nyambura
Kagira, John Maina
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-17
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The detection of Toxoplasma gondii in free-range chickens is a good indicator of possible risk to human beings.
The aim of this study was to investigate the occurrence of T.
gondii in free-range chicken using polymerase chain reaction (PCR).
Brain samples from 105 free-range chickens from three administrative areas in Thika region, Kenya, were collected, DNA-extracted, and analyzed using PCR to detect presence of T.
gondii.
The overall prevalence of T.
gondii in all the three areas was 79.0% (95% CI: 70.0–86.4%) and the prevalence across the three areas was not significantly different ( P = 0.5088 ; χ 2 = 1.354 ).
Female chickens had higher (79.4%) prevalence than males (78.6%), although the difference was not significant ( P = 0.922 , χ 2 = 0.01).
However, chickens that were more than 2 years old had significantly ( P = 0.003 ; χ 2 = 11.87) higher prevalence compared to younger ones.
The study indicates that there was a high occurrence of T.
gondii infection in free-range chickens from Thika region and that the infection rate is age dependent.
Further studies should be carried out to determine the possible role of roaming chickens in the epidemiology of the disease among humans in the area.
American Psychological Association (APA)
Mose, John Mokua& Kagira, John Maina& Karanja, Simon Muturi& Ngotho, J. M.& Kamau, David Muchina& Njuguna, Adele Nyambura…[et al.]. 2016. Detection of Natural Toxoplasma gondii Infection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction. BioMed Research International،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1098824
Modern Language Association (MLA)
Mose, John Mokua…[et al.]. Detection of Natural Toxoplasma gondii Infection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction. BioMed Research International No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1098824
American Medical Association (AMA)
Mose, John Mokua& Kagira, John Maina& Karanja, Simon Muturi& Ngotho, J. M.& Kamau, David Muchina& Njuguna, Adele Nyambura…[et al.]. Detection of Natural Toxoplasma gondii Infection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1098824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098824