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Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting
Joint Authors
Maneg, Daniela
Sponsel, Janina
Müller, Iris
Lohr, Benedikt
Penders, John
Madlener, Katharina
Hunfeld, Klaus P.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Infective endocarditis (IE) is a life-threatening disease that is associated with high morbidity and mortality.
Its long-term prognosis strongly depends on a timely and optimized antibiotic treatment.
Therefore, identification of the causative pathogen is crucial and currently based on blood cultures followed by characterization and susceptibility testing of the isolate.
However, antibiotic treatment starting prior to blood sampling or IE caused by fastidious or intracellular microorganisms may cause negative culture results.
Here we investigate the additional diagnostic value of broad-range PCR in combination with direct sequencing on resected heart tissue or swabs in patients with tissue or swab culture-negative IE in a routine clinical setting.
Sensitivity, specificity, and positive and negative predictive values of broad-range PCR from diagnostic material in our patients were 33.3%, 76.9%, 90.9%, and 14.3%, respectively.
We identified a total of 20 patients (21.5%) with tissue or culture-negative IE who profited by the additional application of broad-range PCR.
We conclude that broad-range PCR on resected heart tissue or swabs is an important complementary diagnostic approach.
It should be seen as an indispensable new tool for both the therapeutic and diagnostic management of culture-negative IE and we thus propose its possible inclusion in Duke’s diagnostic classification scheme.
American Psychological Association (APA)
Maneg, Daniela& Sponsel, Janina& Müller, Iris& Lohr, Benedikt& Penders, John& Madlener, Katharina…[et al.]. 2016. Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098886
Modern Language Association (MLA)
Maneg, Daniela…[et al.]. Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1098886
American Medical Association (AMA)
Maneg, Daniela& Sponsel, Janina& Müller, Iris& Lohr, Benedikt& Penders, John& Madlener, Katharina…[et al.]. Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1098886
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098886