Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
Joint Authors
Escobar-García, Diana María
Aguirre-López, Eva
Méndez-González, Verónica
Pozos-Guillen, Amaury
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
The aim of this study was to evaluate the cytotoxicity and cellular adhesion of Mineral Trioxide Aggregate (MTA) and Biodentine (BD) on periodontal ligament fibroblasts (PDL).
Methods.
PDL cells were obtained from nonerupted third molars and cultured; MTS cellular profusion test was carried out in two groups: MTA and BD, with respective controls at different time periods.
Also, the LIVE/DEAD assay was performed at 24 h.
For evaluation of cellular adhesion, immunocytochemistry was conducted to discern the expression of Integrin β1 and Vinculin at 12 h and 24 h.
Statistical analysis was performed by the Kruskal-Wallis and Mann-Whitney U tests.
Results.
MTA and BD exhibited living cells up to 7 days.
More expressions of Integrin β1 and Vinculin were demonstrated in the control group, followed by BD and MTA, which also showed cellular loss and morphological changes.
There was a significant difference in the experimental groups cultured for 5 and 7 days compared with the control, but there was no significant statistical difference between both cements.
Conclusions.
Neither material was cytotoxic during the time evaluated.
There was an increase of cell adhesion through the expression of focal contacts observed in the case of BD, followed by MTA, but not significantly.
American Psychological Association (APA)
Escobar-García, Diana María& Aguirre-López, Eva& Méndez-González, Verónica& Pozos-Guillen, Amaury. 2016. Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098887
Modern Language Association (MLA)
Escobar-García, Diana María…[et al.]. Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098887
American Medical Association (AMA)
Escobar-García, Diana María& Aguirre-López, Eva& Méndez-González, Verónica& Pozos-Guillen, Amaury. Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098887
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098887