Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
Joint Authors
Hussain, Mohd Hasnain Md.
Chong, Nikson Fatt-Ming
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method.
This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40–45 PCR cycles.
A total of eight mutagenesis experiments were successfully carried out, with each experiment mutating between two to six base pairs.
Up to four adjacent codons were changed in a single experiment.
This method is especially useful for codon optimization, where doublet or triplet rare codons can be changed using a single mutagenic primer set, in a single experiment.
American Psychological Association (APA)
Hussain, Mohd Hasnain Md.& Chong, Nikson Fatt-Ming. 2016. Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098920
Modern Language Association (MLA)
Hussain, Mohd Hasnain Md.& Chong, Nikson Fatt-Ming. Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098920
American Medical Association (AMA)
Hussain, Mohd Hasnain Md.& Chong, Nikson Fatt-Ming. Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098920
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098920