125I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3KAKT Signaling Pathway in HCT116 Cells
Joint Authors
Hu, Lelin
Wang, Hao
Wang, Junjie
Zhao, Yong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
125I seeds brachytherapy implantation has been extensively performed in unresectable and rerecurrent rectal carcinoma.
Many studies on the cancer-killing activity of 125I seeds radiation mainly focused on its ability to trigger apoptosis, which is the most well-known and dominant type of cell death induced by radiation.
However our results showed some unique morphological features such as cell swelling, cytoplasmic vacuolation, and plasma membrane integrity, which is obviously different to apoptosis.
In this study, clonogenic proliferation was carried out to assay survival fraction.
Transmission electron microscopy was used to analyze ultrastructural and evaluate morphologic feature of HCT116 cells after exposure to 125I seeds radiation.
Immunofluorescence analysis was used to detect the origin of cytoplasmic vacuoles.
Flow cytometry analysis was employed to detect the size and granularity of HCT116 cells.
Western blot was performed to measure the protein level of AIP1, caspase-3, AKT, p-Akt (Thr308), p-Akt (Ser473), and β-actin.
We found that 125I seeds radiation activated PI3K/AKT signaling pathway and could trigger paraptosis-like cell death.
Moreover, inhibitor of PI3K/AKT signaling pathway could inhibit paraptosis-like cell death induced by 125I seeds radiation.
Our data suggest that 125I seeds radiation can induce paraptosis-like cell death via PI3K/AKT signaling pathway.
American Psychological Association (APA)
Hu, Lelin& Wang, Hao& Zhao, Yong& Wang, Junjie. 2016. 125I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3KAKT Signaling Pathway in HCT116 Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098984
Modern Language Association (MLA)
Hu, Lelin…[et al.]. 125I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3KAKT Signaling Pathway in HCT116 Cells. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1098984
American Medical Association (AMA)
Hu, Lelin& Wang, Hao& Zhao, Yong& Wang, Junjie. 125I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3KAKT Signaling Pathway in HCT116 Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098984