Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo
Joint Authors
Heo, June Seok
Song, Seung Yong
Lew, Dae Hyun
Choi, Youjeong
Kim, Hyun Ok
Kim, Sinyoung
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) possess great therapeutic potential.
Efficient in vitro expansion of MSCs is however necessary for their clinical application.
The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture.
In this study, we have aimed to improve the functionality and stemness of MSCs during culture using poly-L-lysine (PLL).
Functionality of MSCs was analysed by cell cycle analysis, differentiation assay, β -galactosidase staining, and RT-PCR.
Furthermore, we assessed the global gene expression profile of MSCs on uncoated and PLL-coated plates.
MSCs on PLL-coated plates exhibited a faster growth rate with increased S-phase and upregulated expression of the stemness markers.
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates.
We also confirmed that MSCs on uncoated plates expressed higher β -galactosidase than the MSCs on PLL-coated plates.
We demonstrate that PLL provides favourable microenvironment for MSC culture by reversing the replicative senescence.
This method will significantly contribute to effective preparation of MSCs for cellular therapy.
American Psychological Association (APA)
Heo, June Seok& Kim, Hyun Ok& Song, Seung Yong& Lew, Dae Hyun& Choi, Youjeong& Kim, Sinyoung. 2016. Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo. BioMed Research International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1098997
Modern Language Association (MLA)
Heo, June Seok…[et al.]. Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo. BioMed Research International No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1098997
American Medical Association (AMA)
Heo, June Seok& Kim, Hyun Ok& Song, Seung Yong& Lew, Dae Hyun& Choi, Youjeong& Kim, Sinyoung. Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1098997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098997