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Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots
Joint Authors
Franta, Zdeněk
Vogel, Heiko
Lehmann, Rüdiger
Rupp, Oliver
Goesmann, Alexander
Vilcinskas, Andreas
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-27, 27 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-28
Country of Publication
Egypt
No. of Pages
27
Main Subjects
Abstract EN
Lucilia sericata larvae are used as an alternative treatment for recalcitrant and chronic wounds.
Their excretions/secretions contain molecules that facilitate tissue debridement, disinfect, or accelerate wound healing and have therefore been recognized as a potential source of novel therapeutic compounds.
Among the substances present in excretions/secretions various peptidase activities promoting the wound healing processes have been detected but the peptidases responsible for these activities remain mostly unidentified.
To explore these enzymes we applied next generation sequencing to analyze the transcriptomes of different maggot tissues (salivary glands, gut, and crop) associated with the production of excretions/secretions and/or with digestion as well as the rest of the larval body.
As a result we obtained more than 123.8 million paired-end reads, which were assembled de novo using Trinity and Oases assemblers, yielding 41,421 contigs with an N50 contig length of 2.22 kb and a total length of 67.79 Mb.
BLASTp analysis against the MEROPS database identified 1729 contigs in 577 clusters encoding five peptidase classes (serine, cysteine, aspartic, threonine, and metallopeptidases), which were assigned to 26 clans, 48 families, and 185 peptidase species.
The individual enzymes were differentially expressed among maggot tissues and included peptidase activities related to the therapeutic effects of maggot excretions/secretions.
American Psychological Association (APA)
Franta, Zdeněk& Vogel, Heiko& Lehmann, Rüdiger& Rupp, Oliver& Goesmann, Alexander& Vilcinskas, Andreas. 2016. Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots. BioMed Research International،Vol. 2016, no. 2016, pp.1-27.
https://search.emarefa.net/detail/BIM-1099021
Modern Language Association (MLA)
Franta, Zdeněk…[et al.]. Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots. BioMed Research International No. 2016 (2016), pp.1-27.
https://search.emarefa.net/detail/BIM-1099021
American Medical Association (AMA)
Franta, Zdeněk& Vogel, Heiko& Lehmann, Rüdiger& Rupp, Oliver& Goesmann, Alexander& Vilcinskas, Andreas. Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-27.
https://search.emarefa.net/detail/BIM-1099021
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099021