Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
Joint Authors
Zhang, Siyan
Hughes, Jason D.
Murgolo, Nicholas
Chen, Janice
Liu, Zhong
Shi, Shuangping
Levitan, Diane
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Chinese hamster ovary (CHO) cells have been used widely in the pharmaceutical industry for production of biological therapeutics including monoclonal antibodies (mAb).
The integrity of the gene of interest and the accuracy of the relay of genetic information impact product quality and patient safety.
Here we employed next-generation sequencing, particularly RNA-seq, and developed a method to systematically analyze the mutation rate of the mRNA of CHO cell lines producing a mAb.
The effect of an extended culturing period to mimic the scale of cell expansion in a manufacturing process and varying selection pressure in the cell culture were also closely examined.
American Psychological Association (APA)
Zhang, Siyan& Hughes, Jason D.& Murgolo, Nicholas& Levitan, Diane& Chen, Janice& Liu, Zhong…[et al.]. 2016. Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099035
Modern Language Association (MLA)
Zhang, Siyan…[et al.]. Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099035
American Medical Association (AMA)
Zhang, Siyan& Hughes, Jason D.& Murgolo, Nicholas& Levitan, Diane& Chen, Janice& Liu, Zhong…[et al.]. Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099035