Guitar: An RBioconductor Package for Gene Annotation Guided Transcriptomic Analysis of RNA-Related Genomic Features
Joint Authors
Liu, Hui
Zhang, Lin
Cui, Xiaodong
Huang, Yufei
Meng, Jia
Wei, Zhen
Sun, Lei
Zhang, Shao-Wu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Biological features, such as genes and transcription factor binding sites, are often denoted with genome-based coordinates as the genomic features.
While genome-based representation is usually very effective in correlating various biological features, it can be tedious to examine the relationship between RNA-related genomic features and the landmarks of RNA transcripts with existing tools due to the difficulty in the conversion between genome-based coordinates and RNA-based coordinates.
We developed here an open source Guitar R/Bioconductor package for sketching the transcriptomic view of RNA-related biological features represented by genome based coordinates.
Internally, Guitar package extracts the standardized RNA coordinates with respect to the landmarks of RNA transcripts, with which hundreds of millions of RNA-related genomic features can then be efficiently analyzed within minutes.
We demonstrated the usage of Guitar package in analyzing posttranscriptional RNA modifications (5-methylcytosine and N6-methyladenosine) derived from high-throughput sequencing approaches (MeRIP-Seq and RNA BS-Seq) and show that RNA 5-methylcytosine (m5C) is enriched in 5′UTR.
The newly developed Guitar R/Bioconductor package achieves stable performance on the data tested and revealed novel biological insights.
It will effectively facilitate the analysis of RNA methylation data and other RNA-related biological features in the future.
American Psychological Association (APA)
Cui, Xiaodong& Wei, Zhen& Zhang, Lin& Liu, Hui& Sun, Lei& Zhang, Shao-Wu…[et al.]. 2016. Guitar: An RBioconductor Package for Gene Annotation Guided Transcriptomic Analysis of RNA-Related Genomic Features. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099038
Modern Language Association (MLA)
Cui, Xiaodong…[et al.]. Guitar: An RBioconductor Package for Gene Annotation Guided Transcriptomic Analysis of RNA-Related Genomic Features. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099038
American Medical Association (AMA)
Cui, Xiaodong& Wei, Zhen& Zhang, Lin& Liu, Hui& Sun, Lei& Zhang, Shao-Wu…[et al.]. Guitar: An RBioconductor Package for Gene Annotation Guided Transcriptomic Analysis of RNA-Related Genomic Features. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099038
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099038