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Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation
Joint Authors
Antosik, Paweł
Nowicki, Michał
Zabel, Maciej
Kempisty, Bartosz
Ziolkowska, Agnieszka
Jopek, Karol
Ciesiółka, Sylwia
Budna, Joanna
Bryja, Artur
Kranc, Wiesława
Chachuła, Adrian
Borys, Sylwia
Dyszkiewicz Konwińska, Marta
Bukowska, Dorota
Brüssow, Klaus P.
Bruska, Małgorzata
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Progesterone (P4) and estradiol (E2) play a significant role in mammalian reproduction.
Our study demonstrated that separated porcine cumulus cells (CCs) and/or granulosa cells (GCs) might proliferate in vitro during short-term, real-time primary culture.
The GCs were analyzed according to gene expression of the progesterone receptor (nuclear form) (pgr), progesterone receptor membrane component 1 (pgrmc1), and estrogen-related receptor beta 3 (esrrb3) in relation to two housekeeping genes: actb and pbgd.
GCs were cultivated in medium with the E2.
Both pgr/actb and pgr/pbgd revealed higher expression between 24 and 168 h of IVC of prolonged E2 treatment and at 48 h of IVC after acute E2 administration.
The pgrmc1/actb and pgrmc1/pbgd displayed increased expression after prolonged E2 treatment between 24 and 120 h of IVC.
The highest level of esrrb3/actb at 120 and 144 h, as well as esrrb3/pbgd at 120 h, in untreated controls as compared to the hormone-stimulated group, was observed.
We suggest that E2 significantly influences the upregulation of pgr, pgrmc1, and esrrb3 expression in porcine GCs during real-time cell proliferation.
Since esrrb3 expression is stimulated by E2 in both an acute and prolonged manner, estradiol may be recognized as a potential estrogen receptor agonist in GCs.
American Psychological Association (APA)
Ciesiółka, Sylwia& Budna, Joanna& Jopek, Karol& Bryja, Artur& Kranc, Wiesława& Chachuła, Adrian…[et al.]. 2016. Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099055
Modern Language Association (MLA)
Ciesiółka, Sylwia…[et al.]. Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099055
American Medical Association (AMA)
Ciesiółka, Sylwia& Budna, Joanna& Jopek, Karol& Bryja, Artur& Kranc, Wiesława& Chachuła, Adrian…[et al.]. Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099055