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Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts
Joint Authors
Zheng, Li Wu
Lu, Weijia William
Liu, Xi Ling
Hu, Xiang
Cai, Wei Xin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objectives.
Some animal studies showed that granulocyte-colony stimulating factor (G-CSF) provides beneficial environment for bone healing.
It has been well documented that endothelial cells and osteoblasts play critical roles in multiple phases of bone healing.
However, the biological effects of G-CSF on these cells remain controversial.
This study aimed to investigate the influence of G-CSF at various concentrations on endothelial cells and osteoblasts.
Materials and Methods.
Human umbilical vein endothelial cells (HUVECs) and human osteoblasts (hOBs) were treated with G-CSF at 1000, 100, 10, and 0 ng/mL, respectively.
The capacity of cell proliferation, migration, and tube formation of HUVECs was evaluated at 72, 8, and 6 hours after treatment, respectively.
The capacity of proliferation, differentiation, and mineralization of hOBs was evaluated at 24 hours, 72 hours, and 21 days after treatment, respectively.
Results.
HUVECs treated with 100 and 1000 ng/mL G-CSF showed a significantly higher value comparing with controls in migration assay ( p < 0.001 , p < 0.01 , resp.); the group treated with 1000 ng/mL G-CSF showed a significantly lower value on tube formation.
No significant difference was detected in groups of hOBs.
Conclusions.
G-CSF showed favorable effects only on the migration of HUVECs, and no direct influence was found on hOBs.
American Psychological Association (APA)
Liu, Xi Ling& Hu, Xiang& Cai, Wei Xin& Lu, Weijia William& Zheng, Li Wu. 2016. Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099069
Modern Language Association (MLA)
Liu, Xi Ling…[et al.]. Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099069
American Medical Association (AMA)
Liu, Xi Ling& Hu, Xiang& Cai, Wei Xin& Lu, Weijia William& Zheng, Li Wu. Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099069
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099069