Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin
Joint Authors
Waryah, Charlene Babra
Gogoi-Tiwari, Jully
Wells, Kelsi
Eto, Karina Yui
Masoumi, Elnaz
Costantino, Paul
Kotiw, Michael
Mukkur, Trilochan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
An extensive array of virulence factors associated with S.
aureus has contributed significantly to its success as a major nosocomial pathogen in hospitals and community causing variety of infections in affected patients.
Virulence factors include immune evading capsular polysaccharides, poly-N-acetyl glucosamine, and teichoic acid in addition to damaging toxins including hemolytic toxins, enterotoxins, cytotoxins, exfoliative toxin, and microbial surface components recognizing adhesive matrix molecules (MSCRAMM).
In this investigation, 31 West Australian S.
aureus isolates of human origin and 6 controls were analyzed for relative distribution of virulence-associated genes using PCR and/or an immunoassay kit and MSCRAMM by PCR-based typing.
Genes encoding MSCRAMM, namely, Spa, ClfA, ClfB, SdrE, SdrD, IsdA, and IsdB, were detected in >90% of isolates.
Gene encoding α-toxin was detected in >90% of isolates whereas genes encoding β-toxin and SEG were detectable in 50–60% of isolates.
Genes encoding toxin proteins, namely, SEA, SEB, SEC, SED, SEE, SEH, SEI, SEJ, TSST, PVL, ETA, and ETB, were detectable in >50% of isolates.
Use of RAPD-PCR for determining the virulence factor-based genetic relatedness among the isolates revealed five cluster groups confirming genetic diversity among the MSSA isolates, with the greatest majority of the clinical S.
aureus (84%) isolates clustering in group IIIa.
American Psychological Association (APA)
Waryah, Charlene Babra& Gogoi-Tiwari, Jully& Wells, Kelsi& Eto, Karina Yui& Masoumi, Elnaz& Costantino, Paul…[et al.]. 2016. Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1099112
Modern Language Association (MLA)
Waryah, Charlene Babra…[et al.]. Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1099112
American Medical Association (AMA)
Waryah, Charlene Babra& Gogoi-Tiwari, Jully& Wells, Kelsi& Eto, Karina Yui& Masoumi, Elnaz& Costantino, Paul…[et al.]. Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1099112
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099112