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Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays
Joint Authors
Abdul Rahman, Zaidah
Goay, Yuan Xin
Chin, Kai Ling
Tan, Clarissa Ling Ling
Yeoh, Chiann Ying
Ja’afar, Ja’afar Nuhu
Chinni, Suresh Venkata
Phua, Kia Kien
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Salmonella Typhi (S.
Typhi) causes typhoid fever which is a disease characterised by high mortality and morbidity worldwide.
In order to curtail the transmission of this highly infectious disease, identification of new markers that can detect the pathogen is needed for development of sensitive and specific diagnostic tests.
In this study, genomic comparison of S.
Typhi with other enteric pathogens was performed, and 6 S.
Typhi genes, that is, STY0201, STY0307, STY0322, STY0326, STY2020, and STY2021, were found to be specific in silico.
Six PCR assays each targeting a unique gene were developed to test the specificity of these genes in vitro.
The diagnostic sensitivities and specificities of each assay were determined using 39 S.
Typhi, 62 non-Typhi Salmonella, and 10 non-Salmonella clinical isolates.
The results showed that 5 of these genes, that is, STY0307, STY0322, STY0326, STY2020, and STY2021, demonstrated 100% sensitivity (39/39) and 100% specificity (0/72).
The detection limit of the 5 PCR assays was 32 pg for STY0322, 6.4 pg for STY0326, STY2020, and STY2021, and 1.28 pg for STY0307.
In conclusion, 5 PCR assays using STY0307, STY0322, STY0326, STY2020, and STY2021 were developed and found to be highly specific at single-gene target resolution for diagnosis of typhoid fever.
American Psychological Association (APA)
Goay, Yuan Xin& Chin, Kai Ling& Tan, Clarissa Ling Ling& Yeoh, Chiann Ying& Ja’afar, Ja’afar Nuhu& Abdul Rahman, Zaidah…[et al.]. 2016. Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1099155
Modern Language Association (MLA)
Goay, Yuan Xin…[et al.]. Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1099155
American Medical Association (AMA)
Goay, Yuan Xin& Chin, Kai Ling& Tan, Clarissa Ling Ling& Yeoh, Chiann Ying& Ja’afar, Ja’afar Nuhu& Abdul Rahman, Zaidah…[et al.]. Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1099155
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099155