Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo
Joint Authors
Xue, Gai
Han, Xiaolei
Ma, Xin
Wu, Honghai
Qin, Yabin
Liu, Jianfang
Hu, Yuqin
Hong, Yang
Hou, Yanning
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are considered to be an ideal cell source for cell therapy of many diseases.
The aim of this study was to investigate the contribution of the microenvironment to the hepatic differentiation potential of hUCMSCs in vitro and in vivo and to explore their therapeutic use in acute liver injury in rats.
We established a new model to simulate the liver tissue microenvironment in vivo using liver homogenate supernatant (LHS) in vitro.
This induced environment could drive hUCMSCs to differentiate into hepatocyte-like cells within 7 days.
The differentiated cells expressed hepatocyte-specific markers and demonstrated hepatocellular functions.
We also injected hUCMSCs into rats with CCl4-induced acute hepatic injury.
The hUCMSCs were detected in the livers of recipient rats and expressed the human hepatocyte-specific markers, suggesting that hUCMSCs could differentiate into hepatocyte-like cells in vivo in the liver tissue microenvironment.
Levels of biochemistry markers improved significantly after transplantation of hUCMSCs compared with the nontransplantation group (P<0.05).
In conclusion, this study demonstrated that the liver tissue microenvironment may contribute to the differentiation of hUCMSCs into hepatocytes both in vitro and in vivo.
American Psychological Association (APA)
Xue, Gai& Han, Xiaolei& Ma, Xin& Wu, Honghai& Qin, Yabin& Liu, Jianfang…[et al.]. 2016. Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo. BioMed Research International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1099163
Modern Language Association (MLA)
Xue, Gai…[et al.]. Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo. BioMed Research International No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1099163
American Medical Association (AMA)
Xue, Gai& Han, Xiaolei& Ma, Xin& Wu, Honghai& Qin, Yabin& Liu, Jianfang…[et al.]. Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1099163
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099163