Purification and Characterization of a New Thermostable, Haloalkaline, Solvent Stable, and Detergent Compatible Serine Protease from Geobacillus toebii Strain LBT 77
Joint Authors
Thebti, Wajdi
Riahi, Yosra
Belhadj, Omrane
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A new thermostable, haloalkaline, solvent stable SDS-induced serine protease was purified and characterized from a thermophilic Geobacillus toebii LBT 77 newly isolated from a Tunisian hot spring.
This study reveals the potential of the protease from Geobacillus toebii LBT 77 as an additive to detergent with spectacular proprieties described for the first time.
The protease was purified to homogeneity by ammonium sulfate precipitation followed by Sephadex G-75 and DEAE-Cellulose chromatography.
It was a monomeric enzyme with molecular weight of 30 kDa.
The optimum pH, temperature, and NaCl for maximum protease activity were 13.0, 95°C, and 30%, respectively.
Activity was stimulated by Ca2+, Mg2+, DTNB, β-mercaptoethanol, and SDS.
The protease was extremely stable even at pH 13.25, 90°C, and 30% NaCl and in the presence of hydrophilic, hydrophobic solvents at high concentrations.
The high compatibility with ionic, nonionic, and commercial detergents confirms the utility as an additive to cleaning products.
Kinetic and thermodynamic characterization of protease revealed K m = 1 mg mL−1, V m a x = 217.5 U mL−1, K c a t / K m = 99 mg mL−1 S−1, E a = 51.5 kJ mol−1, and Δ G ⁎ = 56.5 kJ mol−1.
American Psychological Association (APA)
Thebti, Wajdi& Riahi, Yosra& Belhadj, Omrane. 2016. Purification and Characterization of a New Thermostable, Haloalkaline, Solvent Stable, and Detergent Compatible Serine Protease from Geobacillus toebii Strain LBT 77. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099249
Modern Language Association (MLA)
Thebti, Wajdi…[et al.]. Purification and Characterization of a New Thermostable, Haloalkaline, Solvent Stable, and Detergent Compatible Serine Protease from Geobacillus toebii Strain LBT 77. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099249
American Medical Association (AMA)
Thebti, Wajdi& Riahi, Yosra& Belhadj, Omrane. Purification and Characterization of a New Thermostable, Haloalkaline, Solvent Stable, and Detergent Compatible Serine Protease from Geobacillus toebii Strain LBT 77. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099249