The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages
Joint Authors
Xi, Dan
Zhao, Jinzhen
Liu, Jichen
Xiong, Haowei
He, Wenshuai
Hu, Jing
Lai, Wenyan
Guo, Zhigang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Serum amyloid P-component (SAP) contributes to host defense and prevents fibrosis.
Macrophages are the most abundant inflammatory cell type in atherosclerotic plaques.
In the present study, using 3H-cholesterol-labeled counting radioactivity assay, we demonstrated that the apoAI-mediated cholesterol efflux in RAW264.7 macrophages was increased by SAP treatment in a time- and dose-dependent manner.
We analyzed global gene expression changes upon SAP treatment using RNA sequencing.
As a result, a total of 175 differentially expressed genes were identified, of which 134 genes were downregulated and 41 genes were upregulated in SAP treated cells compared to control cells.
Quantitative RT-PCR analysis confirmed decreased expression of 5 genes and an increase in expression of 1 gene upon SAP treatment.
Gene ontology analysis showed that genes involved in response to stimulus were significantly enriched in differentially expressed genes.
Beyond protein-coding genes, we also identified 8 differentially expressed long noncoding RNAs.
Our study may provide new insights into mechanisms underlying the functional role of SAP in macrophages.
American Psychological Association (APA)
Xi, Dan& Zhao, Jinzhen& Liu, Jichen& Xiong, Haowei& He, Wenshuai& Hu, Jing…[et al.]. 2016. The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1099292
Modern Language Association (MLA)
Xi, Dan…[et al.]. The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1099292
American Medical Association (AMA)
Xi, Dan& Zhao, Jinzhen& Liu, Jichen& Xiong, Haowei& He, Wenshuai& Hu, Jing…[et al.]. The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1099292
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099292