Study of Valproic Acid-Enhanced Hepatocyte Steatosis
Joint Authors
Chiu, Chih-Hsien
Chang, Renin
Chou, Mei-Chia
Hung, Li-Ying
Wang, Mu-En
Hsu, Meng-Chieh
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Valproic acid (VPA) is one of the most widely used antiepilepsy drugs.
However, several side effects, including weight gain and fatty liver, have been reported in patients following VPA treatment.
In this study, we explored the molecular mechanisms of VPA-induced hepatic steatosis using FL83B cell line-based in vitro model.
Using fluorescent lipid staining technique, we found that VPA enhanced oleic acid- (OLA-) induced lipid accumulation in a dose-dependent manner in hepatocytes; this may be due to upregulated lipid uptake, triacylglycerol (TAG) synthesis, and lipid droplet formation.
Real-time PCR results showed that, following VPA treatment, the expression levels of genes encoding cluster of differentiation 36 (Cd36), low-density lipoprotein receptor-related protein 1 (Lrp1), diacylglycerol acyltransferase 2 (Dgat2), and perilipin 2 (Plin2) were increased, that of carnitine palmitoyltransferase I a (Cpt1a) was not affected, and those of acetyl-Co A carboxylase α (Acca) and fatty acid synthase (Fasn) were decreased.
Furthermore, using immunofluorescence staining and flow cytometry analyses, we found that VPA also induced peroxisome proliferator-activated receptor γ (PPARγ) nuclear translocation and increased levels of cell-surface CD36.
Based on these results, we propose that VPA may enhance OLA-induced hepatocyte steatosis through the upregulation of PPARγ- and CD36-dependent lipid uptake, TAG synthesis, and lipid droplet formation.
American Psychological Association (APA)
Chang, Renin & Chou, Mei-Chia& Hung, Li-Ying& Wang, Mu-En& Hsu, Meng-Chieh& Chiu, Chih-Hsien. 2016. Study of Valproic Acid-Enhanced Hepatocyte Steatosis. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1099323
Modern Language Association (MLA)
Chang, Renin…[et al.]. Study of Valproic Acid-Enhanced Hepatocyte Steatosis. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1099323
American Medical Association (AMA)
Chang, Renin & Chou, Mei-Chia& Hung, Li-Ying& Wang, Mu-En& Hsu, Meng-Chieh& Chiu, Chih-Hsien. Study of Valproic Acid-Enhanced Hepatocyte Steatosis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1099323
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099323