hsa-miR-376c-3p Regulates Gastric Tumor Growth Both In Vitro and In Vivo
Joint Authors
Tu, Lin
Zhao, Enhao
Zhao, Wenyi
Zhang, Zizhen
Tang, Defeng
Zhang, Yeqian
Wang, Chaojie
Zhuang, Chun
Cao, Hui
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
In recent studies, aberrant expression of various microRNAs (miRNAs) is reported to be associated with gastric cancer metastasis.
Method.
Overexpression construct and inhibitor of hsa-miR-376c-3p were expressed in human gastric adenocarcinoma cell line SGC-7901.
The expression level of tumor related genes was detected by qPCR, western blot, and immunostaining.
Cell apoptosis was determined by flow cytometry.
Xenograft of SGC-7901 cells was used to elucidate the function of hsa-miR-376c-3p in gastric tumor growth in vivo.
Result.
Expression of hsa-miR-376c-3p was detected in SGC-7901 cells.
Downregulation of hsa-miR-376c-3p increased the expression level of BCL-2 and decreased the expression of smad4 and BAD.
On the contrary, overexpression of hsa-miR-376c-3p increased the expression of BAD and smad4, while it led to the decreasing expression level of BCL-2.
Overexpression of hsa-miR-376c-3p also promoted cell apoptosis in vitro and inhibited gastric tumor growth in vivo.
Furthermore, the expression of BCL-2 was higher and expression of smad4 and BAD was lower in tumor tissue than the tissue adjacent to tumor from gastric cancer patients.
Conclusion.
This study demonstrated that hsa-miR-376c-3p plays an important role in the inhibition of gastric tumor growth and tumor related gene expression both in vitro and in vivo.
American Psychological Association (APA)
Tu, Lin& Zhao, Enhao& Zhao, Wenyi& Zhang, Zizhen& Tang, Defeng& Zhang, Yeqian…[et al.]. 2016. hsa-miR-376c-3p Regulates Gastric Tumor Growth Both In Vitro and In Vivo. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1099332
Modern Language Association (MLA)
Tu, Lin…[et al.]. hsa-miR-376c-3p Regulates Gastric Tumor Growth Both In Vitro and In Vivo. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1099332
American Medical Association (AMA)
Tu, Lin& Zhao, Enhao& Zhao, Wenyi& Zhang, Zizhen& Tang, Defeng& Zhang, Yeqian…[et al.]. hsa-miR-376c-3p Regulates Gastric Tumor Growth Both In Vitro and In Vivo. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1099332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099332