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First Observation of Leptospira interrogans in the Lungs of Rattus norvegicus
Joint Authors
Zilber, Anne-Laure
Belli, Patrick
Artois, Marc
Djelouadji, Zoheira
Kodjo, Angeli
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We report the first two cases of pulmonary presence of leptospires in apparently healthy rats captured in a city park in Lyon (France).
Only renal carriage of Leptospira has been described in the literature.
Blood serology was performed in parallel with molecular and histological analyses of the kidney and lung samples.
We isolated leptospires from the kidneys of two out of three seropositive wild rats.
These results were confirmed by specific detection of pathogenic Leptospira by real-time PCR.
Moreover, Leptospira DNA was detected in lung tissues.
Immunohistochemistry and Warthin-Starry staining revealed that leptospires were present on the surface of the ciliated epithelium of the bronchi.
Using PCR of the rrs (16S) gene and Multispacer Sequence Typing, DNA extracts of the kidney and lung were identified as belonging to Leptospira interrogans serovar Icterohaemorrhagiae “CHU Réunion.” This first observation of the presence Leptospira in the lung with simultaneous renal carriage will require further study in future on several target organs to gain a better understanding of the Leptospira infection in wild rat.
American Psychological Association (APA)
Zilber, Anne-Laure& Belli, Patrick& Artois, Marc& Kodjo, Angeli& Djelouadji, Zoheira. 2016. First Observation of Leptospira interrogans in the Lungs of Rattus norvegicus. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099341
Modern Language Association (MLA)
Zilber, Anne-Laure…[et al.]. First Observation of Leptospira interrogans in the Lungs of Rattus norvegicus. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099341
American Medical Association (AMA)
Zilber, Anne-Laure& Belli, Patrick& Artois, Marc& Kodjo, Angeli& Djelouadji, Zoheira. First Observation of Leptospira interrogans in the Lungs of Rattus norvegicus. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099341