Periostin Facilitates the Epithelial-Mesenchymal Transition of Endometrial Epithelial Cells through ILK-Akt Signaling Pathway
Joint Authors
Lu, Jingjing
Zheng, Qiao-mei
Zhao, Jing
Wei, Xuan
Wang, Lu
Liu, Pei-shu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Although periostin was confirmed to facilitate the pathogenesis of endometriosis by enhancing the migration, invasion, and adhesion of human endometrial stromal cells (ESCs), its effect on the endometrial epithelial cells (EECs) is still unknown.
The current study aimed to determine whether periostin enhanced the epithelial-mesenchymal transition (EMT) of EECs.
EECs were isolated from 12 women with endometriosis.
The migration and invasion abilities of EECs were evaluated by transwell assays.
Expressions of proteins were detected by western blot.
After treatment with periostin, the migration and invasion abilities of EECs were enhanced.
Additionally, E-cadherin and keratin were downregulated while N-cadherin and vimentin were upregulated in EECs.
Simultaneously, levels of ILK, p-Akt, slug, and Zeb1 were all upregulated in EECs.
After silencing the expression of ILK in EECs, levels of p-Akt, slug, Zeb1, N-cadherin, and vimentin were downregulated while E-cadherin and keratin were upregulated.
Although periostin weakened the above effects in EECs after silencing the expression of ILK, it failed to induce the EMT of EECs.
Thus, periostin enhanced invasion and migration abilities of EECs and facilitated the EMT of EECs through ILK-Akt signaling pathway.
Playing a pivotal role in the pathogenesis of endometriosis, periostin may be a new clinical therapy target for endometriosis.
American Psychological Association (APA)
Zheng, Qiao-mei& Lu, Jingjing& Zhao, Jing& Wei, Xuan& Wang, Lu& Liu, Pei-shu. 2016. Periostin Facilitates the Epithelial-Mesenchymal Transition of Endometrial Epithelial Cells through ILK-Akt Signaling Pathway. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099414
Modern Language Association (MLA)
Zheng, Qiao-mei…[et al.]. Periostin Facilitates the Epithelial-Mesenchymal Transition of Endometrial Epithelial Cells through ILK-Akt Signaling Pathway. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099414
American Medical Association (AMA)
Zheng, Qiao-mei& Lu, Jingjing& Zhao, Jing& Wei, Xuan& Wang, Lu& Liu, Pei-shu. Periostin Facilitates the Epithelial-Mesenchymal Transition of Endometrial Epithelial Cells through ILK-Akt Signaling Pathway. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099414
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099414