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Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry
Joint Authors
Sharma, Bechan
Singh, Pramod Kumar
Shrivastava, Nidhi
Chaturvedi, Krishna
Bhagyawant, Sameer S.
Source
Biochemistry Research International
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Proteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions.
Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE) across a broad range 3.0–10.0 immobilized pH gradient (IPG) strips.
Comparative elucidation of differentially expressed proteins between two diverse geographically originated chickpea accessions was carried out using 2D-GE coupled with mass spectrometry.
A total of 600 protein spots were detected in these accessions.
In-gel protein expression patterns revealed three protein spots as upregulated and three other as downregulated.
Using trypsin in-gel digestion, these differentially expressed proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) which showed 45% amino acid homology of chickpea seed storage proteins with Arabidopsis thaliana.
American Psychological Association (APA)
Singh, Pramod Kumar& Shrivastava, Nidhi& Chaturvedi, Krishna& Sharma, Bechan& Bhagyawant, Sameer S.. 2016. Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry. Biochemistry Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1099463
Modern Language Association (MLA)
Singh, Pramod Kumar…[et al.]. Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry. Biochemistry Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1099463
American Medical Association (AMA)
Singh, Pramod Kumar& Shrivastava, Nidhi& Chaturvedi, Krishna& Sharma, Bechan& Bhagyawant, Sameer S.. Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry. Biochemistry Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1099463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099463