Presynaptic Proteins as Markers of the Neurotoxic Activity of BmjeTX-I and BmjeTX-II Toxins from Bothrops marajoensis (Marajó Lancehead) Snake Venom
Joint Authors
Rodrigues-Simioni, Lea
Rocha, Thalita
Lisboa, Antonio
Melaré, Rodolfo
Franco, Junia R. B.
Bis, Carolina V.
Gracia, Marta
Ponce Soto, L. A.
Marangoni, Sérgio
da Cruz-Höfling, Maria Alice
Source
Biochemistry Research International
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Neuromuscular preparations exposed to B.
marajoensis venom show increases in the frequency of miniature end-plate potentials and twitch tension facilitation followed by presynaptic neuromuscular paralysis, without evidences of muscle damage.
Considering that presynaptic toxins interfere into the machinery involved in neurotransmitter release (synaptophysin, synaptobrevin, and SNAP25 proteins), the main objective of this communication is to analyze, by immunofluorescence and western blotting, the expression of the synaptic proteins, synaptophysin, synaptobrevin, and SNAP25 and by myography, light, and transmission electron microscopy the pathology of motor nerve terminals and skeletal muscle fibres of chick biventer cervicis preparations (CBC) exposed in vitro to BmjeTX-I and BmjeTX-II toxins from B.
marajoensis venom.
CBC incubated with toxins showed irreversible twitch tension blockade and unaffected KCl- and ACh-evoked contractures, and the positive colabelling of acetylcholine receptors confirmed that their action was primarily at the motor nerve terminal.
Hypercontraction and loose myofilaments and synaptic vesicle depletion and motor nerve damage indicated that the toxins displayed both myotoxic and neurotoxic effect.
The blockade resulted from interference on synaptophysin, synaptobrevin, and SNAP25 proteins leading to the conclusion that BmjeTX-I and BmjeTX-II affected neurotransmitter release machinery by preventing the docking of synaptic vesicles to the axolemma of the nerve terminal.
American Psychological Association (APA)
Lisboa, Antonio& Melaré, Rodolfo& Franco, Junia R. B.& Bis, Carolina V.& Gracia, Marta& Ponce Soto, L. A.…[et al.]. 2016. Presynaptic Proteins as Markers of the Neurotoxic Activity of BmjeTX-I and BmjeTX-II Toxins from Bothrops marajoensis (Marajó Lancehead) Snake Venom. Biochemistry Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1099469
Modern Language Association (MLA)
Lisboa, Antonio…[et al.]. Presynaptic Proteins as Markers of the Neurotoxic Activity of BmjeTX-I and BmjeTX-II Toxins from Bothrops marajoensis (Marajó Lancehead) Snake Venom. Biochemistry Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1099469
American Medical Association (AMA)
Lisboa, Antonio& Melaré, Rodolfo& Franco, Junia R. B.& Bis, Carolina V.& Gracia, Marta& Ponce Soto, L. A.…[et al.]. Presynaptic Proteins as Markers of the Neurotoxic Activity of BmjeTX-I and BmjeTX-II Toxins from Bothrops marajoensis (Marajó Lancehead) Snake Venom. Biochemistry Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1099469
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099469