Differences in GPR30 Regulation by Chlorotriazine Herbicides in Human Breast Cells
Joint Authors
Schultz-Norton, Jennifer R.
Florian, Colin P.
Mansfield, Shelly R.
Source
Biochemistry Research International
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Over 200,000 cases of invasive breast cancer are diagnosed annually; herbicide contaminants in local water sources may contribute to the growth of these cancers.
GPR30, a G protein coupled receptor, was identified as a potential orphan receptor that may interact with triazine herbicides such as atrazine, one of the most commonly utilized chlorotriazines in agricultural practices in the United States.
Our goal was to identify whether chlorotriazines affected the expression of GPR30.
Two breast cancer cell lines, MDA-MB-231 and MCF-7, as well as one normal breast cell line, MCF-10A, were treated with a 100-fold range of atrazine, cyanazine, or simazine, with levels flanking the EPA safe level for each compound.
Using real-time PCR, we assessed changes in GPR30 mRNA compared to a GAPDH control.
Our results indicate that GPR30 expression increased in breast cancer cells at levels lower than the US EPA drinking water contamination limit.
During this treatment, the viability of cells was unaltered.
In contrast, treatment with chlorotriazines reduced the expression of GPR30 in noncancerous MCF-10A cells.
Thus, our results indicate that cell milieu and potential to metastasize may play a role in the extent of GPR30 response to pesticide exposure.
American Psychological Association (APA)
Florian, Colin P.& Mansfield, Shelly R.& Schultz-Norton, Jennifer R.. 2016. Differences in GPR30 Regulation by Chlorotriazine Herbicides in Human Breast Cells. Biochemistry Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1099474
Modern Language Association (MLA)
Florian, Colin P.…[et al.]. Differences in GPR30 Regulation by Chlorotriazine Herbicides in Human Breast Cells. Biochemistry Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1099474
American Medical Association (AMA)
Florian, Colin P.& Mansfield, Shelly R.& Schultz-Norton, Jennifer R.. Differences in GPR30 Regulation by Chlorotriazine Herbicides in Human Breast Cells. Biochemistry Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1099474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099474