Production and Characterization of Highly Thermostable β-Glucosidase during the Biodegradation of Methyl Cellulose by Fusarium oxysporum
Joint Authors
Ogunyewo, Olusola A.
Olajuyigbe, Folasade M.
Nlekerem, Chidinma M.
Source
Biochemistry Research International
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Production of β-glucosidase from Fusarium oxysporum was investigated during degradation of some cellulosic substrates (Avicel, α-cellulose, carboxymethyl cellulose (CMC), and methylcellulose).
Optimized production of β-glucosidase using the cellulosic substrate that supported highest yield of enzyme was examined over 192 h fermentation period and varied pH of 3.0–11.0.
The β-glucosidase produced was characterized for its suitability for industrial application.
Methyl cellulose supported the highest yield of β-glucosidase (177.5 U/mg) at pH 6.0 and 30°C at 96 h of fermentation with liberation of 2.121 μmol/mL glucose.
The crude enzyme had optimum activity at pH 5.0 and 70°C.
The enzyme was stable over broad pH range of 4.0–7.0 with relative residual activity above 60% after 180 min of incubation.
β-glucosidase demonstrated high thermostability with 83% of its original activity retained at 70°C after 180 min of incubation.
The activity of β-glucosidase was enhanced by Mn2+ and Fe2+ with relative activities of 167.67% and 205.56%, respectively, at 5 mM and 360% and 315%, respectively, at 10 mM.
The properties shown by β-glucosidase suggest suitability of the enzyme for industrial applications in the improvement of hydrolysis of cellulosic compounds into fermentable sugars that can be used in energy generation and biofuel production.
American Psychological Association (APA)
Olajuyigbe, Folasade M.& Nlekerem, Chidinma M.& Ogunyewo, Olusola A.. 2016. Production and Characterization of Highly Thermostable β-Glucosidase during the Biodegradation of Methyl Cellulose by Fusarium oxysporum. Biochemistry Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099481
Modern Language Association (MLA)
Olajuyigbe, Folasade M.…[et al.]. Production and Characterization of Highly Thermostable β-Glucosidase during the Biodegradation of Methyl Cellulose by Fusarium oxysporum. Biochemistry Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099481
American Medical Association (AMA)
Olajuyigbe, Folasade M.& Nlekerem, Chidinma M.& Ogunyewo, Olusola A.. Production and Characterization of Highly Thermostable β-Glucosidase during the Biodegradation of Methyl Cellulose by Fusarium oxysporum. Biochemistry Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099481
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099481