Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes
Joint Authors
Source
Biochemistry Research International
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The promastigote stage of Leishmania resides in the sand fly gut, enriched with sugar molecules.
Recently we reported that Leishmania donovani possesses a sucrose uptake system and a stable pool of intracellular sucrose metabolizing enzyme.
In the present study, we purified the intracellular sucrase nearly to its homogeneity and compared it with the purified extracellular sucrase.
The estimated size of intracellular sucrase is ~112 kDa by gel filtration chromatography, native PAGE, and substrate staining.
However, in SDS-PAGE, the protein is resolved at ~56 kDa, indicating the possibility of a homodimer in its native state.
The kinetics of purified intracellular sucrase shows its higher substrate affinity with a K m of 1.61 mM than the extracellular form having a K m of 4.4 mM.
The highly specific activity of intracellular sucrase towards sucrose is optimal at pH 6.0 and at 30°C.
In this report the purification and characterization of intracellular sucrase provide evidence that sucrase enzyme exists at least in two different forms in Leishmania donovani promastigotes.
This intracellular sucrase may support further intracellular utilization of transported sucrose.
American Psychological Association (APA)
Singh, Arpita& Mandal, Debjani. 2016. Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes. Biochemistry Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099490
Modern Language Association (MLA)
Singh, Arpita& Mandal, Debjani. Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes. Biochemistry Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099490
American Medical Association (AMA)
Singh, Arpita& Mandal, Debjani. Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes. Biochemistry Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099490