Isolation and identification of methicillin resistance staphylococcus aureus and detection their ability to the production of virulence factors

Other Title(s)

عزل و تشخيص بكتريا المكورات العنقودية الذهبية Staphylococcus aureus المقاومة للمثيسلين Methicillin و الكشف عن قابليتها على إنتاج عوامل الضراوة

Author

al-Khafaji, Angham Najah

Source

Journal of Babylon University : Journal of Applied and Pure Sciences

Issue

Vol. 26, Issue 8 (31 Aug. 2018), pp.100-111, 12 p.

Publisher

University of Babylon

Publication Date

2018-08-31

Country of Publication

Iraq

No. of Pages

12

Main Subjects

Biology

Abstract EN

Objectives: Isolation and Identification of S.aureus and study their susceptibility to the production of virulence factors.

* Methods: A total 50 clinical specimens of Staphylococcus aureus isolates were collected in AL- Najaf AL- Asharaf governorate during the period of (10/10/2013-20/1/2014) by the cultural characteristic colonies, microscopic for cells, biochemical tests, antibiotics susceptibility test to Staphylococcus aureus using an antibiotic diffusion disc assay, in addition to studying the virulence factors produced from S.aureus .

* Results: The results show that the Staphylococcus aureus resistant to methicillin formed 70% and 30% of isolates were sensitive to the same antibiotics.

Tested 10 isolates to methicillin resistant for their efficiency in forming the highest inhibitory areas for testing their sensitivity to other antibiotics used, the results show S.aureus resistant to penicillin about 100%, while there is no resistance to CO-Trimethoprim because it was sensitive completly 100%.

The susceptibility investigated of Staphylococcus aureus resistance methicillin isolates to the production of biofilm and slime layer with using both tube method and growth on Congo red agar respectively.

Ten isolates of S.aureus were tested based on resistance to methicillin and their high susceptibility to the production of the biofilm and the slime layer.

* Conclusion: So we conclusion, they have Staphylococcus aureus is highly resistant to β- Lactam antibiotics and the CO-trimethoprim is the most effective in the inhibiting growth of this bacteria.

In addition to its ability to produce virulence factors.

The increase cause bacterial resistance to our local isolates may be due to the frequent use of antibiotics, which allowed for increased bacterial resistance to various antibiotics.

American Psychological Association (APA)

al-Khafaji, Angham Najah. 2018. Isolation and identification of methicillin resistance staphylococcus aureus and detection their ability to the production of virulence factors. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 8, pp.100-111.
https://search.emarefa.net/detail/BIM-1100395

Modern Language Association (MLA)

al-Khafaji, Angham Najah. Isolation and identification of methicillin resistance staphylococcus aureus and detection their ability to the production of virulence factors. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 8 (2018), pp.100-111.
https://search.emarefa.net/detail/BIM-1100395

American Medical Association (AMA)

al-Khafaji, Angham Najah. Isolation and identification of methicillin resistance staphylococcus aureus and detection their ability to the production of virulence factors. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 8, pp.100-111.
https://search.emarefa.net/detail/BIM-1100395

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 108-110

Record ID

BIM-1100395