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Antibacterial activity of streptomyces sp. isolated from high tolerant ecosystem in Babylon province, Iraq
Author
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 26, Issue 7 (31 Jul. 2018), pp.303-308, 6 p.
Publisher
Publication Date
2018-07-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
The goal of this research is producing antibiotic from two Actynomycites species isolated from the high tolerant ecosystem, isolate from high salty soil and high alkaloid soil, used whey and yeast extract medium as a perfect medium to culturing (1% glucose, 1% trypton, 0.092% glycine, 0.004% HCl and 8.5 PH), perfect incubation circumstances are 30 C0 in 4 days, the infiltrate of crude antibiotic concentrate by using a rotary evaporator, then extract the antibiotic from concentrate infiltrate with ethanol: methanol: ethyl acetate methods ( 50:30:20 Vol), drying the extract at 30 C0 to dissolve both antibiotic in distilled water with three concentration ( 50, 75, 100 μg/ml) and disinfectant by 0.45 Millipore.
Antibiotic activity from two isolates of Streptomyces sp.
were measured against positive and negative control bacteria (Escherichia coli, Bacillus subtilis, streptococcus pyogenes and staphylococcus aureus) by well diffusion method, the results show the antibiotic produced by isolating No.
2 active against E.
coli, S.
aureus and S.
pyogenes while the isolating No.
1 were active only against E.
coli and no activity against gram positive bacteria.
Extract component was separated with TLC technique (thin layer chromatography) by using silica gel 20*20cm with high polar mix (7 water: 5 acetic acid: 3 biotanol vol.) according to relative factor Rf for antibiotic, isolate No.1 give 0.6 Rf for one antibiotic and isolate No.2 give 0.15 Rf for two antibiotic against S.
aureus and the other one with 0.45 Rf against E.
coli and S.
pyogenes, means these antibiotic has synergism activity.
American Psychological Association (APA)
al-Mawla, Yasir H.. 2018. Antibacterial activity of streptomyces sp. isolated from high tolerant ecosystem in Babylon province, Iraq. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 7, pp.303-308.
https://search.emarefa.net/detail/BIM-1100931
Modern Language Association (MLA)
al-Mawla, Yasir H.. Antibacterial activity of streptomyces sp. isolated from high tolerant ecosystem in Babylon province, Iraq. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 7 (2018), pp.303-308.
https://search.emarefa.net/detail/BIM-1100931
American Medical Association (AMA)
al-Mawla, Yasir H.. Antibacterial activity of streptomyces sp. isolated from high tolerant ecosystem in Babylon province, Iraq. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 7, pp.303-308.
https://search.emarefa.net/detail/BIM-1100931
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 307-308
Record ID
BIM-1100931