![](/images/graphics-bg.png)
Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer
Joint Authors
Skrypkina, Inessa
Tsyba, Liudmyla
Onyshchenko, Kateryna
Morderer, Dmytro
Kashparova, Olena
Nikolaienko, Oleksii
Panasenko, Grigory
Vozianov, Sergii
Romanenko, Alina
Rynditch, Alla
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The critical point for successful treatment of cancer is diagnosis at early stages of tumor development.
Cancer cell-specific methylated DNA has been found in the blood of cancer patients, indicating that cell-free DNA (cfDNA) circulating in the blood is a convenient tumor-associated DNA marker.
Therefore methylated cfDNA can be used as a minimally invasive diagnostic marker.
We analysed the concentration of plasma cfDNA and methylation of six tumor suppressor genes in samples of 27 patients with renal cancer and 15 healthy donors as controls.
The cfDNA concentrations in samples from cancer patients and healthy donors was measured using two different methods, the SYBR Green I fluorescence test and quantitative real-time PCR.
Both methods revealed a statistically significant increase of cfDNA concentrations in cancer patients.
Hypermethylation on cfDNA was detected for the LRRC3B (74.1%), APC (51.9%), FHIT (55.6%), and RASSF1 (62.9%) genes in patients with renal cancer.
Promoter methylation of VHL and ITGA9 genes was not found on cfDNA.
Our results confirmed that the cfDNA level and methylation of CpG islands of RASSF1A, FHIT, and APC genes in blood plasma can be used as noninvasive diagnostic markers of cancer.
American Psychological Association (APA)
Skrypkina, Inessa& Tsyba, Liudmyla& Onyshchenko, Kateryna& Morderer, Dmytro& Kashparova, Olena& Nikolaienko, Oleksii…[et al.]. 2016. Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer. Disease Markers،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1103692
Modern Language Association (MLA)
Skrypkina, Inessa…[et al.]. Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer. Disease Markers No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1103692
American Medical Association (AMA)
Skrypkina, Inessa& Tsyba, Liudmyla& Onyshchenko, Kateryna& Morderer, Dmytro& Kashparova, Olena& Nikolaienko, Oleksii…[et al.]. Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1103692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103692